Journal: Lab on a chip

Article Title: A microfluidic array with cellular valving for single cell co-culture.

doi: 10.1039/c0lc00172d

Figure Lengend Snippet: Fig. 6 Heterotypic single cell co-culture. Unlabelled single SW480 cells co-cultured with single SW480 cells metabolically labelled with the fluorescent product of calcein AM (A). A single cell couple (B), and the same couple immunostained with connexin 43 (red, C). A trap : cell diameter ratio of $2.0 was required for efficient (70%) cell coupling (D).

Article Snippet: Human SW480 epithelial, HT29 colon carcinoma and MCF-7 epithelial-like breast cancer cells were purchased from DSMZ (Germany).

Techniques: Co-Culture Assay, Cell Culture, Metabolic Labelling

Journal: Cancer Science

Article Title: PC‐407, a celecoxib derivative, inhibited the growth of colorectal tumor in vitro and in vivo

doi: 10.1111/j.1349-7006.2009.01335.x

Figure Lengend Snippet: Effects of celecoxib or PC‐407 (4‐[5‐naphthyl‐3‐(trifluoromethyl)‐1H‐pyrazol‐1‐yl] benzenesulfonamide) on the growth of SW‐1116, HT‐29, and SW‐480 cells. (a) Celecoxib and PC‐407 inhibited cell growth of colorectal cancer cell lines in a dose‐dependent way. All the three cell lines were treated with different concentrations of celecoxib or PC‐407 for 72 h. (b) Celecoxib and PC‐407 inhibited cell growth of colorectal cancer cell lines in a time‐dependent manner. All the three cell lines were treated with 30 μM of celecoxib or PC‐407. Cells were counted every 24 h. Values are the mean ± SD of three independent experiments.

Article Snippet: Human colorectal cancer cell lines (SW‐1116, HT‐29, and SW‐480) were obtained from the American Type Culture Collection.

Techniques:

Journal: Cancer Science

Article Title: PC‐407, a celecoxib derivative, inhibited the growth of colorectal tumor in vitro and in vivo

doi: 10.1111/j.1349-7006.2009.01335.x

Figure Lengend Snippet: PC‐407 (4‐[5‐naphthyl‐3‐(trifluoromethyl)‐1H‐pyrazol‐1‐yl] benzenesulfonamide) induced apoptosis of colorectal cancer (CRC) cells in a dose‐dependent way. SW‐1116, HT‐29, and SW‐480 cells were incubated with or without PC‐407 (15 or 30 μM) for 48 h. (a) DNA ladder formation. DNA samples were extracted and electrophoresed to detect DNA fragmentation; (b) flow cytometric analysis for apoptosis of human colorectal cancer cells. Q1 showed that cells were undergoing necrosis, and Q2 showed that cells were at the end stage of apoptosis. Q3 showed that cells were viable, or there was no measurable apoptosis. Q4 showed that cells were undergoing apoptosis.

Article Snippet: Human colorectal cancer cell lines (SW‐1116, HT‐29, and SW‐480) were obtained from the American Type Culture Collection.

Techniques: Incubation

Journal: Cancer Science

Article Title: PC‐407, a celecoxib derivative, inhibited the growth of colorectal tumor in vitro and in vivo

doi: 10.1111/j.1349-7006.2009.01335.x

Figure Lengend Snippet: The effects of celecoxib and PC‐407 (4‐[5‐naphthyl‐3‐(trifluoromethyl)‐1H‐pyrazol‐1‐yl] benzenesulfonamide) on the mRNA level of cyclooxygenase‐2 (COX‐2), and the protein level of COX‐2, COX‐1, Bcl‐2, Bax, caspase‐3, ‐8, ‐9, phospho‐GSK‐3β, β‐catenin, and phospho‐β‐catenin in colorectal cancer (CRC) cells or azoxymethane/dextran sodium sulfate (AOM/DSS)‐treated ICR mice. (a) Total RNA was collected after the cell lines exposed to 15 or 30 μM of celecoxib or PC‐407 for 48 h and RT‐PCR was employed to detect the expression of COX‐2. (b) Western blot analysis of Bcl‐2, Bax, caspase‐3, ‐8, ‐9, phospho‐GSK‐3β, β‐catenin, and phospho‐β‐catenin expressions after treatments of SW‐1116, HT‐29, and SW‐480 cells with 15 or 30 μM of celecoxib or PC‐407 for 48 h or of ICR mice with 0.04% celecoxib or 0.02% PC‐407 for 5 weeks.

Article Snippet: Human colorectal cancer cell lines (SW‐1116, HT‐29, and SW‐480) were obtained from the American Type Culture Collection.

Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Western Blot

Journal: Cancer Science

Article Title: PC‐407, a celecoxib derivative, inhibited the growth of colorectal tumor in vitro and in vivo

doi: 10.1111/j.1349-7006.2009.01335.x

Figure Lengend Snippet: Immunofluorescent staining for cyclooxygenase‐2 (COX‐2) in the mouse colitis‐associated colorectal cancer (CACC) model. Scale bar, 100 μm (a) Control group; (b) model group; (c) celecoxib group. (d) PC‐407 (4‐[5‐naphthyl‐3‐(trifluoromethyl)‐1H‐pyrazol‐1‐yl] benzenesulfonamide) group (×20).

Article Snippet: Human colorectal cancer cell lines (SW‐1116, HT‐29, and SW‐480) were obtained from the American Type Culture Collection.

Techniques: Staining, Control

Journal: Cancer Science

Article Title: PC‐407, a celecoxib derivative, inhibited the growth of colorectal tumor in vitro and in vivo

doi: 10.1111/j.1349-7006.2009.01335.x

Figure Lengend Snippet: The pathological finding in the colon in the mouse colitis‐associated colorectal cancer (CACC) model. Scale bar, 100 μm (a) Control group; (b) model group, adenoma and obvious glandular proliferation. (c) Celecoxib group, inflammatory cells infiltrate in mucosa. (d) PC‐407 (4‐[5‐naphthyl‐3‐(trifluoromethyl)‐1H‐pyrazol‐1‐yl] benzenesulfonamide) group, inflammatory cells infiltrate in mucosa and edema in submucosa of the colon (×10).

Article Snippet: Human colorectal cancer cell lines (SW‐1116, HT‐29, and SW‐480) were obtained from the American Type Culture Collection.

Techniques: Control

Journal: Oncology letters

Article Title: MicroRNA-539 inhibits colorectal cancer progression by directly targeting SOX4.

doi: 10.3892/ol.2018.8892

Figure Lengend Snippet: Figure 1. miR‑539 was downregulated in CRC tissues and cell lines. (A) The expression of miR‑539 was detected in 49 pairs of CRC tissues and corre sponding normal adjacent tissues by reverse transcription‑quantitative polymerase chain reaction. *P<0.05 vs. normal adjacent tissues. (B) Relative expression levels of miR‑539 in five CRC cell lines (LoVo, HCT116, HT29, SW480 and SW620) and the normal human colon epithelium cell line FHC. *P<0.05 vs. the FHC. miR‑539, microRNA‑539; CRC, colorectal cancer.

Article Snippet: A total of five human CRC cell lines (LoVo, HCT116, HT29, SW480 and SW620) and the normal human colon epithelium cell line FHC were purchased from the American Type Culture Collection (Manassas, VA, USA).

Techniques: Expressing, Polymerase Chain Reaction

Journal: Oncology letters

Article Title: MicroRNA-539 inhibits colorectal cancer progression by directly targeting SOX4.

doi: 10.3892/ol.2018.8892

Figure Lengend Snippet: Figure 5. SOX4 rescued the inhibitory effects of miR‑539 mimics on HCT116 and SW480 cell proliferation and invasion. (A) Western blot analysis of SOX4 protein expression in HCT116 and SW480 cells transfected with pcDNA3.1‑SOX4 or pcDNA3.1. (B) Cell proliferation was assessed by MTT assay. (C) Invasion was assessed by Transwell assay (x200 magnifica tion). *P<0.05 vs. miR‑NC and miR‑539 mimics+pcDNA3.1‑SOX4. SOX4, SRY‑box 4; miR‑539, microRNA‑539; NC, negative control. Figure 4. SOX4 was upregulated in CRC tissues and was negatively corre lated with miR‑539 expression levels. (A) Expression of SOX4 mRNA was assessed in 49 pairs of CRC tissues and corresponding normal adjacent tissues. *P<0.05 vs. normal adjacent tissues. (B) SOX4 protein expression was determined in CRC tissues and normal adjacent tissues by western blotting. (C) There was a significant negative correlation between miR‑539 and SOX4 mRNA expression levels in CRC tissues. SOX4, SRY‑box 4; CRC, colorectal cancer; miR‑539, microRNA‑539.

Article Snippet: A total of five human CRC cell lines (LoVo, HCT116, HT29, SW480 and SW620) and the normal human colon epithelium cell line FHC were purchased from the American Type Culture Collection (Manassas, VA, USA).

Techniques: Western Blot, Expressing, Transfection, MTT Assay, Transwell Assay, Negative Control

Journal: Clinical Cancer Research

Article Title: Deregulated GSK3β Sustains Gastrointestinal Cancer Cells Survival by Modulating Human Telomerase Reverse Transcriptase and Telomerase

doi: 10.1158/1078-0432.ccr-09-0973

Figure Lengend Snippet: Fig. 1. Expression, phosphorylation, and activity of GSK3β in cell lines. A, protein extracts from HEK293 cells and colon (SW480, HCT116, HT-29), stomach (MKN-28, NKPS, TMK-1, NUGC-4), pancreatic (MIAPaCa-2, Capan-1, BxPC-3), and liver (HepG2) cancer cells were analyzed by Western immunoblotting for GSK3β expression and levels of pGSK3βS9, pGSK3βY216, and β-actin. B, GSK3β activity was detected by NRIKA (24) in HEK293 cells and the cancer cell lines indicated above. GSK3β was isolated by immunoprecipitation from 1 mg aliquot of cells lysate from each sample. An in vitro kinase reaction was then carried out in the presence of immunoprecipitated GSK3β, its substrate of recombinant human β-catenin protein (β-cateninHis), and nonradioisotopic ATP in the kinase reaction buffer. The resultant products were analyzed by Western immunoblotting for phosphorylation of β-cateninHis

Article Snippet: Human embryonic kidney cells (HEK293) and colon (SW480, HCT116, HT-29), pancreatic (MIAPaCa-2, Capan-1, BxPC-3) and liver (HepG2) cancer cell lines were obtained from the American Type Culture Collection.

Techniques: Expressing, Phospho-proteomics, Activity Assay, Western Blot, Isolation, Immunoprecipitation, In Vitro, Recombinant

Journal: Oncology Letters

Article Title: Effect of lidocaine and ropivacaine on primary (SW480) and metastatic (SW620) colon cancer cell lines

doi: 10.3892/ol.2019.10332

Figure Lengend Snippet: Cell viability in SW480 and SW620 following incubation with increasing concentrations of ropivacain are presented. (A) Ropivacaine increases cell viability in SW480 after 24 h at 500 and 1,000 µM. (B) Ropivacaine increases cell viability in SW480 after 48 h at 500 µM. A decrease was observed at 1,000 µM. (C) Ropivacaine reduces cell viability in SW480 after 72 h at 1,000 µM. (D) Ropivacaine increases cell viability in SW620 after 24 h at 1,000 µM. (E) After 48 h a significant increase in cell viability in SW620 is noted in all concentrations except 1,000 µM. (F) Ropivacaine increases cell viability in SW620 after 72 h at concentrations between 5 and 50 µM, a reduction in cell viability was observed at 1,000 µM. Results are presented as the median ratio and range of CellTiter Blue® values from three independent experiments in quadruplicate (n=12) between drug exposed cells and unexposed cells (control=1, dotted line). *P<0.05 vs. control.

Article Snippet: Immortalized human colon cancer cells SW480 and SW620 were purchased from American Type Culture Collection (ATCC ® CCL-228 and CCL-227).

Techniques: Incubation

Journal: Oncology Letters

Article Title: Effect of lidocaine and ropivacaine on primary (SW480) and metastatic (SW620) colon cancer cell lines

doi: 10.3892/ol.2019.10332

Figure Lengend Snippet: Cell viability in SW480 and SW620 after incubation with increasing concentrations of lidocaine is presented. (A) Lidocaine increases cell viability in SW480 after 24 h at 5 and 500 µM. (B and C) No significant change in cell viability in SW480 is seen after 48 and 72 h with lidocaine. (D) Lidocaine increases cell viability in SW620 after 24 h at 1,000 µM. (E) After 48 h an increase in cell viability in SW620 is noted at all tested concentrations. (F) Lidocaine increases cell viability in SW620 after 72 h at concentrations between 10 and 50 µM, and at 500 µM. Results are presented as the median ratio and range of Celltiter Blue® values from three independent experiments in quadruplicate (n=12) between drug exposed cells and unexposed cells (control=1, dotted line). *P<0.05 vs. control.

Article Snippet: Immortalized human colon cancer cells SW480 and SW620 were purchased from American Type Culture Collection (ATCC ® CCL-228 and CCL-227).

Techniques: Incubation

Journal: Oncology Letters

Article Title: Effect of lidocaine and ropivacaine on primary (SW480) and metastatic (SW620) colon cancer cell lines

doi: 10.3892/ol.2019.10332

Figure Lengend Snippet: Cell proliferation measured by PKH67 is presented. The results are presented as the median ratio and range of PKH67 values from three independent experiments in duplicate (n=6) between drug exposed cells and drug-free cells (control=1, dotted line). (A) Lidocaine significantly decreased cell proliferation in SW480 at 1,000 µM. (B) Ropivacaine did not significantly affect cell proliferation in SW480 at any concentration tested. (C) Lidocaine demonstrated no significant effect on cell proliferation in SW620 at any concentration tested. (D) Ropivacaine significantly decreased cell proliferation in SW620 at 1,000 µM. *P<0.05 vs. control.

Article Snippet: Immortalized human colon cancer cells SW480 and SW620 were purchased from American Type Culture Collection (ATCC ® CCL-228 and CCL-227).

Techniques: Concentration Assay

Journal: Oncology Letters

Article Title: Effect of lidocaine and ropivacaine on primary (SW480) and metastatic (SW620) colon cancer cell lines

doi: 10.3892/ol.2019.10332

Figure Lengend Snippet: Change in cell proliferation measured by PKH67 presented in overlays. The data are presented as an overlay histogram of PKH67 median fluorescence intensity compared with the drug-free control (0 µM) and 1,000 µM L or R. (A) Overlay of PKH67 median fluorescence intensity for SW480 cells comparing drug-free control (light grey) and 1,000 µM L (black). (B) Overlay of PKH67 median fluorescence intensity for SW480 cells comparing drug free control (light grey) and 1,000 µM R (black). (C) Overlay of PKH67 median fluorescence intensity for SW620 cells comparing drug-free control (light grey) and 1,000 µM L (black). (D) Overlay of PKH67 median fluorescence intensity for SW620 cells comparing drug free control (light grey) and 1,000 µM R (black). L, lidocaine; R, ropivacaine.

Article Snippet: Immortalized human colon cancer cells SW480 and SW620 were purchased from American Type Culture Collection (ATCC ® CCL-228 and CCL-227).

Techniques: Fluorescence